WebA) Recombinant luciferase was serially diluted in 1X Firefly Lysis Buffer with 1 Mg/mL BSA and measured in the assay. B) The Firefly Luciferase HTS Assay is a steady-glow high … WebSep 22, 2024 · The luciferase reporter assay is commonly used as a tool to study gene expression at the transcriptional level. It is widely used because it is convenient, relatively inexpensive, and gives quantitative …

Bioluminescence for in vivo detection of cell-type-specific ...

WebJun 3, 2015 · The conventional methods for microbial viability quantification require cultivation and are laborious. There is consequently a widespread need for cultivation-free methods. The adenosine triphosphate (ATP) bioluminescence-sensing assay is considered an extremely effective biosensor; hence ATP is the energy currency of all … WebATP bioluminescence. ATP releasing agent (Somalyze), Tris-acetate buffer, ATP-monitoring reagent (firefly luciferase, D-luciferin) and ATP-standard (EG+G. Wallac, Turku, Finland) are needed for the assay. 100 µL of ATP releasing agent was added to each well of a 96 well microtitre tissue culture plate (Greiner, Germany), containing 100 µL of ... hays county car title transfer

Bioluminescence for in vivo detection of cell-type-specific ...

WebAug 7, 2014 · GFP (Green Fluorescent Protein) Applications of Bioluminescence • The protein, GFP can be found in the photo organs of Aequorea victoria. • In this certain process, the blue light is absorbed by … WebThe ATP Bioluminescence Assay Kit HS II is specifically developed for the high-sensitivity detection of ATP. Due to the high concentration of luciferase in the assay, the reaction exhibits a peak kinetic. If ATP determinations are performed to obtain kinetic data of the enzymes involved ( e.g., for metabolic studies or if coupled enzymatic ... WebBioluminescence assay has a large range of applications, such as cytotoxicity test [83], in situ evaluation of the impact of biofilms in situ, and drug screening on Leishmania. It has been demonstrated that this technique can provide results in 3–5 days for antimycobacterial tests [84,85] in comparison with the conventional dilution technique ... bottom fishing rigs for salmon