Peasy‐uni seamless cloning and assembly kit
WebpEASY®-T1 Cloning Kit is designed for cloning and sequencing Taq-amplified PCR products. Pricing & Availability Download Catalogue Add to wishlist. ... pEASY® -Uni Seamless Cloning and Assembly Kit. No restriction enzyme digestions, > 95% cloning efficiency, and no extra sequences introduced. WebSep 24, 2024 · The pEASY-Uni Seamless Cloning and Assembly Kit (TransGen Biotech) was used to construct different recombinogenic DNA fragments according to the …
Peasy‐uni seamless cloning and assembly kit
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Webexperiments were performed with pEASY®-Uni Seamless Cloning and assembly kit (TransGen Biotech, Beijing, China). Construction of formolase error-prone (epPCR) library and screening epPCR libraries were generated by the standard epPCR method using variant formolase (fls) gene as template (21). For the mutagenic PCR (95 ºC for 2 min, 1 cycle ... WebSeamless cloning methods, such as co-transformation cloning, sequence- and ligation-independent cloning (SLIC) or the Gibson assembly, are essential tools for the precise construction of...
WebSep 29, 2024 · The fragments were ligated using the pEASY-Uni Seamless Cloning and Assembly Kit, and the constructed plasmid was transformed into AsCEP112 mutant protoplasts using PEG-mediated protoplast transformation technology. Finally, neomycin resistance was used to screen for revertant strains. Melanin, penetration and … WebJul 4, 2016 · The digested fragments of pK18 mobsacB, P tac -iMCS, and rrnBT1T2 -iMCS were mixed and supplemented with pEASY ® -Uni Seamless Cloning and Assembly Kit (TRANSGEN, Beijing, China) to assemble these fragments (Fig. 1 ). After incubation at 50 °C for 15 min, the mixture was electroporated into E. coli JM109 competent cells, and spread …
WebFeatures of the pEASY-Uni Seamless Cloning and Assembly Kit Fast assembly and fast cloning: 15 minutes Easy: regardless of restriction enzyme sites of inserts, and … WebDNA fragments were PCR amplified with Q5 DNA polymerase, purified using the EZ-10 Spin Column PCR Products Purification Kit (Bio-Basic, Canada) and assembled either using the pEASY-Uni Seamless Cloning and Assembly Kit (TransGen Biotech, China), following manufacturer’s instructions, or by E. coli mediated assembly [21].
WebDec 1, 2024 · Recently a commercially available pEASY-Uni Seamless Cloning and Assembly Kit (TransGen Biotech) has been made based on the technology of Gibson …
WebThermo Fisher peasy uni seamless cloning assembly kit Peasy Uni Seamless Cloning Assembly Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: … new movies 2021 for the familyWebNov 25, 2024 · They were all constructed using the pEASY-Uni Seamless Cloning and Assembly Kit (TransGene Biotech, China), which can be used to recombine the vector linearized by any method and the PCR fragment with 15–25bp overlapping region with both ends of the linearized vector. new movies 2021 horror out nowWebJul 1, 2024 · For fragment assembly, the mixture was mixed with the 2× Assembly Mix solution (pEASY-Uni Seamless Cloning and Assembly Kit, Transgen, pEASY®-Uni Seamless Cloning and Assembly Kit uses a proprietary enzyme mix to recognize and precisely assemble DNA fragments sharing end homology. The enzyme mix includes DNA 5′ … introducing motion trackingWebOct 13, 2024 · To increase protein solubility, four TwCPR ORFs were subcloned into the N-terminal MBP fusion expression vector HIS-MBP-pET28a according to the protocol of the pEASY® -Uni Seamless Cloning and Assembly Kit (TransGen Biotech; HIS-MBP-pET28a was provided by Dr. Xiaohong Zhang. HIS, histidine; MBP, maltose-binding protein. new movies 2021 horrorWebApr 12, 2024 · The subsequent ligation reaction was carried out at 50 °C according to the pEASY-Basic Seamless Cloning and Assembly Kit (TransGen Biotech), after which ligation products were transformed into E. coli DH5α. Positive colonies were screened and plasmid was isolated and sent to Sangon (Shanghai, China) for DNA sequencing. Protein purification new movies 2021 hindi southWebNov 25, 2024 · They were all constructed using the pEASY-Uni Seamless Cloning and Assembly Kit (TransGene Biotech, China), which can be used to recombine the vector linearized by any method and the PCR fragment with 15–25bp overlapping region with both ends of the linearized vector. All the fragments needed for chloroplast expression vector … introducing mrWebPostdoctoral researcher at Tallinn University of Technology - Cited by 193 - CRISPR/dCas9 Gene Regulation - Genetic Engineering - Molecular Biology - Plant Bioinformatics ... Evaluation of pEASY-Uni Seamless Cloning and Assembly Kit to clone multiple fragments of Elaeis guineensis DNA. M Moradpour, SNA ... new movies 2021 in hindi