Pylex1
WebAdditional file 1: Table S1. Primers used for the construction of the heterologous CoA-dependent pathway. Figure S1. GC/MS detection of 1-butanol production in two Y. lipolytica engineered strains carrying the chromosomally integrated plasmid pYLEX1-All-GPD or pYLEX1-All-MDH. Figure S2. Map of the plasmids pYLEX1, pYLEX1-2 and pYLEX1-3. … WebSnapGene Viewer is free software that allows molecular biologists to create, browse, and share richly annotated sequence files. Gain unparalleled visibility of your plasmids, DNA …
Pylex1
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Web在本发明的另一些具体实施方案中，所述菌株的构建方法为：以耶氏解脂酵母ATCC201249为底盘菌株，在不敲除ERG5的基础上，利用整合型质粒pYLEX1在底盘菌基因组上整合所述外源功能基因。. 本发明还提供了所述的菌株在发酵生产菜油甾醇中的应用。. 本发明还提供 ... WebMar 2, 2024 · Non-homologous end-joining (NHEJ)-mediated random integration in Yarrowia lipolytica has been demonstrated to be an effective strategy for screening hyperproducer …
WebpVAX1 is specifically designed for use in the development of DNA vaccines. pVAX1 has the following features:Eukaryotic DNA sequences limited to those required for expression in order to minimize the possibility of chromosomal integr WebCreates a new instance of the Map class and initializes it by copying the entries from the specified map. T1 is the data type of the keys and T2 is the data type of the values. …
WebJul 2, 2024 · The original plasmid pYLEX1, a pBR322-based monocopy integrative vector [12, 36], is produced by Yeastern Biotech Co., Ltd. Y. lipolytica Po1g KU70Δ was fitted … WebY˜ et al. Microb Cell Fact 0 7 P610 deletionofYALI0E17787gcanactivate1-butanolpro-ductionisthatthealcoholdehydrogenaseencodedby ...
Webreplacing the hp4d promoter of pYLEX1 with the trans-lation elongation factor-1a (TEF) promoter as described in the literature [18]. This promoter was synthesized (Genscript) as a SalI-KpnI fragment and ligated into the appropriately restricted pYLEX1 vector. The gene encod-ing Maqu_2220, a fatty acyl-CoA reductase (MaFAR)
WebDec 27, 2024 · P. brasilianum hmfL1 is also heterologously expressed in the yeast Yarrowia lipolytica Polg using pYLEX1-hmfL1 vector and conferred the ability to produce FDCA from HMF. This transformant was checked for activity in glucose-containing mineral medium supplemented with 4 mM HMF. boston bruins hockey schedule for 2021 seasonWebPubMed hawkeye boy scout councilWebNov 22, 2013 · YLEX Expression Kit based on INRA INAPG licensed patent provides an easy approach for cloning and expressing a gene of interest in the yeast, Yarrowia lipolytica . Using this kit, high level of heterologous protein may be expressed intracellularly or be secreted from the cell into medium by selecting the supplied expression vector pYLEX1 … boston bruins hockey socks youthWebPlasmid pLH01 from Dr. Sheng Yang's lab contains the insert PorfX-Lp_0640-42, sapK-sappR and is published in Biotechnol J. 2024 Jul;14(7):e1800690. doi: … hawkeye breeders service iowaWebNov 23, 2024 · It is loaded into an automated matrix sprayer (TM Sprayer, HTX Imaging) that coats the printed well substrate with a layer of DHB matrix at 60 °C using a flow rate of 0.125 mL/min and velocity of ... hawkeye boys bb scheduleWebJun 18, 2011 · Amplification of the tyrosinase gene (melO) from the genomic DNA of Aspergillus oryzae NCIM 1212 yielded a 1.6-kb product. This gene was cloned into pYLEX1, and the resulting pTyro-YLEX1 vector was transformed in Yarrowia lipolytica strain Po1g. A clone displaying the highest specific activity for tyrosinase (10.94 U/mg) was used for … hawkeye breeders phonehttp://www.biovector.net/product/2293675.html hawkeye breeders service inc