Tmem192-rfp-3xha
WebTo isolate the lysosomes, antihemagglutinin (anti-HA) magnetic beads were used to pull down the Tmem192-3xHA–lysosome cells (HA-Lyso cells), as described by Abu-Remaileh et al. 15 Briefly, 1 × 10 7 HA-Lyso HEK-293T cells cultured on 15-cm plates (with or without PPI treatment) were used for each lysosomal immunoprecipitation (LysoIP) assay. WebTMEM192 is a transmembrane protein (8) that we find retains its lysosomal localization upon overexpression better than other such proteins, like lysosomal-associated …
Tmem192-rfp-3xha
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WebOct 26, 2024 · Tmem192-3xHA and lysosomes were detected by immunofluorescence with antibodies to the HA epitope tag and the lysosomal marker LAMP2, respectively. ... (r 2 = 0.95; Fig. 1G) and even with those obtained using the less preferable LAMP1-RFP-3xHA as the lysosomal antigen tag (r 2 = 0.95; Fig. S1C), mitigating concerns that expression of … WebOct 26, 2024 · Tmem192-3xHA and lysosomes were detected by immunofluorescence with antibodies to the HA epitope tag and the lysosomal marker LAMP2, respectively. Scale …
WebAll lanes : Anti-TMEM192 antibody [EPR14330-67] (ab186737) at 1/10000 dilution Lane 1 : U87-MG cell lysate Lane 2 : Human fetal liver lysate Lane 3 : Human fetal lung lysate Lysates/proteins at 20 µg per lane. Secondary All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution Predicted band size: 31 kDa Observed band size: … WebJan 3, 2024 · We performed centrifugation at 20,000 × g to generate an organelle-enriched pellet, two-step sucrose density gradient centrifugation, enrichment by …
WebDisclosed herein are methods and compositions for modulating MFSD12 expression and activity to treat diseases such as lysosomal storage diseases, including cystinosis. Also disclosed are methods of altering skin pigmentation and methods of screening for MFSD12 modulation agents. WebOct 7, 2024 · Briefly, HeLa TMEM192-3xHA endogenously tagged cells that were grown to 80% confluency in 150-mm plates were washed twice with PBS, scraped into tubes, and then pelleted at 500×g for 5 min.
WebJan 3, 2024 · We performed centrifugation at 20,000 × g to generate an organelle-enriched pellet, two-step sucrose density gradient centrifugation, enrichment by superparamagnetic iron oxide nanoparticles (SPIONs), and immunoprecipitation using a 3xHA tagged version of the lysosomal membrane protein TMEM192.
WebMar 29, 2024 · TMEM192 transmembrane protein 192 [ (human)] Gene ID: 202431, updated on 29-Mar-2024. Summary. Enables protein homodimerization activity. Located in several … synthetic oil in an older engineWebMar 21, 2024 · TMEM219 (Transmembrane Protein 219) is a Protein Coding gene. Diseases associated with TMEM219 include Hermansky-Pudlak Syndrome . Among its related … synthetic oil hazmatWeb> 无血清快速细胞冻存液. QuickFreezing-M. QuickFreezing-2*M synthetic oil how far between oil changesWebGSC#9 stably expressing the lysosomal protein TMEM192 tagged with either 3xHA (HA-lyso) or 2xFLAG (FLAG-lyso) were mechanically lysed. Lysosomes were immunoprecipitated with anti-HA magnetic beads. These immunopurified lysosome fractions were subsequently analyzed by western-blot and label-free quantification (LFQ) proteomic. thames flood warning areaWebApr 1, 2024 · Cells with endogenously-tagged TMEM192 and stably expressing FLAG-EEA1 are referred to as 293 EL cells, for E ndo-IP and L yso-IP. These cells were also prepared in a form that has a deletion of the APP gene (293 EL APP -/- ) and the same cells reconstituted with a lentivirus stably expressing a patient mutant form (APP Sw;T700N ) which ... synthetic oil how often to changeWebproduct name : pLJM1-Tmem192-mRFP-3xHA catalog : 134631 more info or order : Addgene product webpage citations: 2 product information Catalog Number : 134631 … thamesfield schoolWebAug 13, 2024 · HA-TMEM192-isolated lysosomes represented a highly pure fraction as determined by the absence of markers for other cellular compartments such as … synthetic oil life expectancy